FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12565466
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA45850.016.8715821
GTATAAG26800.016.208391
GTATAGA28400.015.7142871
GTATATA47000.015.699131
GTATTAT45150.015.6834271
TATACTG42800.015.570665
GTATTAG29550.015.5054721
GTATAGG20000.015.471241
CTATACT28600.014.7715744
GTGTAGA47750.014.7048431
TATACCG8100.014.6918925
TACACTG53200.014.6518495
TAATACT50950.014.3646634
TAGACAG52250.014.3488465
GTCTTAC37850.013.9918751
GTGTAGG44350.013.9537681
CTAGACT33650.013.61570554
TTATACT56450.013.492134
TAGGGCG13450.013.2718585
GTATTGG36050.013.2049421
Produced by FastQC (version 0.11.5)