FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12587372
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG8000.019.353845
TAGACAG51800.016.669485
GTATAGG19850.016.1998181
TATACCG8100.016.174155
CTAGGAC38100.015.0050333
GTATTAA43150.014.9045831
GTATAGA34250.014.77866651
ATACCGT12900.014.7663986
CTATACT28100.014.6228994
TATACTG43450.014.5278015
GTATATA50400.014.17841151
CTAGACA43200.014.0607064
ATAGGAC29250.014.0479823
CTAGACT32050.013.9355444
GTCCTAG34200.013.9296671
GTATTAG28300.013.8878291
GTCCTAC30600.013.8170011
TCTAGAC33600.013.6471563
TATACAG40750.013.5906165
ATAGTAC22800.013.5818593
Produced by FastQC (version 0.11.5)