FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12662998
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG14100.017.3024625
CTATACT27350.016.5348174
GTATAAG29600.016.4844931
TATACTG40750.016.208345
GTATTAA43950.015.9763141
GTGTAGG46950.015.8426511
GTATAGA31200.015.8298551
GTATTAT45400.015.7281931
GTATATA50500.015.55378151
GTCTAAG29800.015.3754521
CCTAGAC33200.015.0552443
TAGGGTG32500.015.0132135
TATACAG42800.014.8758965
CTAGTAC16950.014.7443683
GTATTAC30500.014.6323771
TAGACAG50850.014.6272345
GCTATAC21200.014.5953353
GTATAGG20200.014.4343811
GTATTAG26900.014.1573221
GTGTATA37800.014.0104361
Produced by FastQC (version 0.11.5)