FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12569870
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT37900.018.2119454
GTATTAG37700.018.1516671
TATACTG54150.016.372765
GTATAAG35750.016.1456571
GTATAGA40800.016.0432571
GTGTAGA56000.015.6202991
GTATTAA62750.015.552141
TCTATAC43350.015.5106633
TACACCG18300.015.2820245
GTATAGG25150.015.1426531
TACACTG58600.015.0278975
GTATTAT63050.014.7231081
GCTATAC25850.014.5017713
GTATATA69200.014.3605271
GTATAAT55400.014.2857121
TATACCG9200.014.2288235
CTAGACT39750.014.0711074
TATACAG56950.013.791595
CTATACA46750.013.7461064
CTAATAC43200.013.6361713
Produced by FastQC (version 0.11.5)