FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12630939
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT37550.017.2851434
TATACTG54200.016.918995
GTGTACG24400.016.5948031
TAATACT67600.016.0317574
GTCCTAG37900.015.7113591
TAGACAG60500.015.3540245
CTAGACT37350.015.1457034
CTAATAC40550.014.8315643
TATACAG53800.014.8311735
GTATTAG38300.014.7699091
CCTAGAC37000.014.484293
TATACCG9500.014.4164135
GTATTAT61900.014.3333791
GTATAGG27200.013.7919171
GTCTAGG38250.013.6994851
GTATAAG37550.013.6377111
CTAGGAC41350.013.3925673
TACACTG61900.013.3714075
GTATATA67200.013.202921
GTGCTAG38650.013.095511
Produced by FastQC (version 0.11.5)