FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12687447
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG35900.017.5693261
TATACAG53250.016.4262335
GTGTACG22100.016.4240881
GTATAAG38600.016.0320741
GTATATA65250.015.502871
TACACCG18650.015.3144955
GTATTAT65200.015.1497061
GTATACG8300.015.0551421
TAGACCG10300.015.0202235
GTACTAG20250.014.9861071
TATACTG53650.014.9728445
GTCTTAG41300.014.8398961
GTCTAGA41700.014.55485251
GTATAAT55650.014.4348511
CTATACT36800.014.39071754
TACACTG62200.014.3496445
GTGTAGG52550.014.1540591
CTAGACT40700.013.8889514
GTATAGA42600.013.8283161
GTATAGG25850.013.8112941
Produced by FastQC (version 0.11.5)