FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12687447
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG18050.016.8295575
CTATACT38750.016.2943824
TATACTG51100.016.2021875
GTATATA68100.015.3945091
GTATAGG25650.014.8625351
GTATTAG36700.014.2829171
GTATAGA43350.013.7407751
GTCCTAG39950.013.7173861
GTACGAC18350.013.6338013
ATAATAC61900.013.5685113
GTATTAA62800.013.5636541
TATACAG54100.013.542155
TAATACT65550.013.1764694
CTAGACT36650.013.1648174
TATATAC68900.013.140993
CCTAGAC35850.013.126283
GTGTATA53400.013.0510331
TACACTG63750.012.8937325
TAGACAG61950.012.8837775
TAATACA74750.012.8297574
Produced by FastQC (version 0.11.5)