FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7636238
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG8350.021.3781095
GTATTAG17100.017.0510651
GTCCTAG19000.016.9118731
GTCTAGA19500.016.4782351
GTATTAT31900.016.4151021
GTATAGA20550.015.6362831
CTACACT23400.015.0027434
GTATAAA41700.014.5551171
GTGTAAG25350.014.5534281
GTATACG4505.9611557E-714.5456031
GTGTAGA29100.014.5183391
CTAATAC21100.014.3820983
GTATATA33150.014.3601191
GTATAAG19150.014.2935671
GTCCTAT16350.014.1937911
CTATACT16450.014.1069544
TATAGTG17500.013.9405635
GTATAGG13750.013.8483761
TAGAGGT24950.013.8322224
GTCTTAA25700.013.6606221
Produced by FastQC (version 0.11.5)