FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7636238
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA35550.017.760771
TACACCG8550.017.4153565
GTCCTAG19200.016.4425871
GTATAGG13600.016.2053471
CTAGACT17300.015.8372134
GTATTAA30550.015.7931711
GTATTAG16100.015.5388531
GTATAAA37850.014.635671
GTACTAA15100.014.5955451
TAGACTG27750.014.5950115
GTATTAC18900.014.4974481
GTGTTAG21850.014.4484061
TATACTG27550.014.0523915
CTAACAC23000.013.7250863
GTTCTAG21950.013.5684741
GTCTTAG18450.013.55964851
TCTAGAC19950.013.4349683
GTATAAG20550.013.333421
GTGTACG10350.013.23681
TTAGACT24750.013.2359314
Produced by FastQC (version 0.11.5)