FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22854881
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG13550.016.2635735
GTATTAG72350.016.0537411
CTATACT73450.015.8124174
GTACTAG41100.015.7966461
GTATAGA81550.015.26521
TCTATAC77500.015.0629423
TATACTG109850.014.8018535
CTACACT95950.014.6494884
GTATATA145900.014.6153071
TACACTG131950.014.5796355
GTATTAA117300.014.5735081
GTATAGG53200.014.3310681
TAGACTG94050.014.0587825
GTATTAT120500.014.0382021
GTCCTAG83150.013.7536881
TAGACAG129750.013.6333515
CCTATAC57400.013.4892223
TATACAG118950.013.4191165
CTAGACT74150.013.2534284
GTGTATA117400.012.9882931
Produced by FastQC (version 0.11.5)