FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23051577
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG71800.016.657761
GTATAGA77500.016.4307381
TATACTG104050.016.0122535
GTATAGG55600.015.5180871
GTATATA145400.015.5102841
GTCCTAG82950.015.42293551
TACACTG134450.015.4012135
TATACAG106500.015.0851865
TAATACT133800.014.6755484
GTATAAG71100.014.311051
GTATTAT121350.013.7297691
ATAATAC124200.013.6540553
GTACTAG41100.013.6091511
GTATATG105500.013.592821
TTATACT139000.013.5700364
TATACCG15000.013.4872735
CTAGACT79300.013.4312484
GTGTTAG90950.013.4120811
CTACACT96600.013.3665434
TAGACTG93250.013.3362575
Produced by FastQC (version 0.11.5)