FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24572261
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA126600.017.6727071
GTATATA162600.017.639061
GTATTAT133400.016.638031
GTATAAT125450.015.9374021
TATACTG117400.015.8642295
ATAATAC139850.015.5301553
CTATACT80350.015.033284
GTATAGA83900.014.9647481
GTATAGG55550.014.461011
TATACCG15350.014.342875
GTATTAG77450.014.2902691
GTATAAG78350.014.2780121
GTATAAA177800.014.190021
TAATACT144900.014.126474
TATACAG121400.014.0671445
CTAATAC85800.014.0090753
ATTATAC129750.013.8957073
TCTATAC85450.013.6486413
GTGTATA112650.013.6281671
TAATACA174350.013.0030934
Produced by FastQC (version 0.11.5)