FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24674270
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA161800.017.7020341
GTATTAT134700.017.0638221
GTATTAG82800.016.5407091
ATAATAC134600.016.4574133
CTATACT78800.016.1715344
TATACTG115500.015.8277185
TATACAG120750.015.0409275
GTATAAT124550.015.0121371
GTATAGG59650.014.4748531
TAATACT144650.014.2848184
GTATAAG78450.014.0422081
TATACTA87650.013.9272015
GTACTAT67800.013.876631
GTATTAA130650.013.8554551
TTATACT148500.013.8342714
CCTATAC57900.013.7812923
ATTATAC129000.013.7097773
GTATAAA179950.013.5009681
GTATAGA85100.013.29476451
TAATACA172600.013.2136154
Produced by FastQC (version 0.11.5)