FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13695011
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG31550.016.9748061
GTATAGA36800.016.8169481
GTATATA61000.016.5836731
GTACGAC14750.016.1366653
TACACCG14000.015.725935
GTGTACG18650.015.6343161
CTATACT33650.015.5612124
GTATTAA56300.015.5371251
GTATAGT25700.015.281731
TATACTG48350.015.2604685
GTCTAGG34350.015.0714211
GTGTAGG47850.014.5500871
TAGACAG63350.014.3710155
CTAGACT35150.014.2200054
GTACTAG19250.014.21964551
GTCCTAA37300.014.1985121
GTGTATA42450.014.1581131
TACACTG61450.014.1375325
TACGACA18700.014.000934
CTAGGAC41700.013.9841423
Produced by FastQC (version 0.11.5)