FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13695011
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG5550.018.245014
CTAGACT32800.017.433544
GTGTACG21000.017.3034271
CTATACT31900.016.805064
TAGACAG58900.016.483745
TATACTG52500.016.1106285
GTATATA61250.015.85269451
GTCCTAG37350.015.4704421
GTATTAG33700.015.2016291
GTATAAG34600.015.1505421
GTGTTAG40950.014.9832111
GTATACG7700.014.6988881
CCTAGAC34950.014.6568113
TAGACTG50400.014.5364395
CTACACT43100.014.5110964
TAGACCG9050.014.4796325
GGACCGT21250.014.2857246
TCTAGAC37850.014.1633123
TCTATAC36450.014.0536493
TATACAG50900.014.0425795
Produced by FastQC (version 0.11.5)