FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13806950
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG30150.018.749261
GTATAGG24800.017.7552951
GTGTACG20550.017.6630571
CTATACT32150.017.5825164
GTACGAC14550.017.1761683
CTACACT38450.016.713444
GTATATA61750.016.5744571
GTGCTAG37300.016.2719351
TATACTG50050.016.0497445
GTATAGA33200.015.9514351
TATACCG6800.015.7507785
GTATTAA56250.015.6562251
GTATTAT56100.015.3798811
TACACCG12850.015.280915
TAGACAG58350.015.1944095
TAGACCG9850.015.1022685
TATAGTG30950.014.9958945
CCTAGAC36050.014.8551573
CTAGACT35100.014.7485924
ATAATAC56600.014.6129673
Produced by FastQC (version 0.11.5)