FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19523297
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT56350.018.2672924
TATACTG83550.017.590275
TATACCG11500.017.0741125
TAATACT102450.016.9587124
CCTATAC41800.016.3698063
GTATAGG41150.016.0504971
GTGTACG31550.016.0307881
TATACAG86600.015.3217725
GTATTAG54850.014.7535881
CTAATAC58700.014.6977853
TACACCG22050.014.5715975
TACACTG98600.014.0001615
TAGACAG102250.013.8495495
GTATAGA59450.013.8121911
CTACACT76350.013.7938744
GTATAAG58150.013.7116731
ATAATAC97350.013.6909643
GTGTATA77900.013.6725941
GTATATA107300.013.4199521
TCTATAC60350.013.3100143
Produced by FastQC (version 0.11.5)