FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21481142
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG87700.017.9786625
CTATACT62750.017.06754
TCTATAC62650.016.8098283
GTATTAG59600.015.5753921
TATACAG90200.015.5674165
GTATAGG43000.015.0840751
GTATATA108600.014.8490991
CTATACG9650.014.7977274
ATAATAC100750.014.7640683
TTATACT111050.014.1983674
TAATACT108050.014.0969864
TAGACAG113800.013.9598275
CTACACT80650.013.8696194
CTAGACT67400.013.8596054
CTAGGAC76450.013.8533123
TACACTG110000.013.6848155
TAGACCG12200.013.6555535
CTTACAC83800.013.27726653
TACACCG22700.013.105555
GTGTATA80500.013.0839241
Produced by FastQC (version 0.11.5)