FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21481142
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN240020.11173521407753834No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT61400.017.560754
TATACTG89850.016.5083625
GTATAGG41250.015.5978611
GTATATA114750.015.0041161
CTAGACT68450.014.8818024
GTATTAG57300.014.86781
CTACACT82200.014.7839744
CCTAGAC67300.014.7818983
TAGACAG110400.014.6225435
TCTATAC63600.014.3305583
TACACTG118050.014.2804915
GTATTAT98850.014.2233081
GTATAGA62950.014.0065541
TAGACCG14200.013.8435765
GTATTAA104750.013.7065541
GTATTAC59200.013.5855531
TATACAG94550.013.5456445
TTATACT119500.013.5093424
GTACTAG34400.013.5083231
GTATAAT97250.013.3546391
Produced by FastQC (version 0.11.5)