FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21434475
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT58950.017.1591534
TATACTG86450.016.2434315
CTACACT76450.015.2548984
TATACAG92900.014.859465
TACACTG107200.014.7644615
GTATAGG42950.014.5471571
TAATACT104900.014.3507894
CTAGGAC79850.014.3073463
CTAATAC63000.014.1671813
GTATATA119300.013.9160441
TTATACT117950.013.8223974
CTAGACT70250.013.7214314
TCTATAC59450.013.7120223
ATAATAC100300.013.4072683
CTAGTAC33800.013.3791863
TAGACAG110600.013.2345965
CCTACAC85000.013.2304813
TATACTA65300.013.1213975
TACACAG148050.013.0618795
TATAGTG57600.013.0160375
Produced by FastQC (version 0.11.5)