FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21434475
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN231270.10789627457635423No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT59800.018.0261614
TATACTG89500.015.6376215
GTATTAG61150.015.4858721
GTACTAG33950.014.9112531
TCTATAC61900.014.8168523
GTATAAG57900.014.8121791
CTAGGAC78700.014.7565823
TACACTG111600.014.7289155
TAGACTG85450.014.4969665
GTATAGA61900.014.3360861
CTACACT79900.014.311344
TACACCG21650.014.3044555
GTATATA113300.013.92999651
TAGACAG106350.013.8320115
GTGTACG36600.013.6688891
GTCTTAG65450.013.6494951
TATACAG92850.013.5981495
GTATTAT99000.013.5357491
GTATTAA104500.013.5072531
TAGGACT94550.013.4796344
Produced by FastQC (version 0.11.5)