FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20308064
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN231390.1139399600080047No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT52500.017.1350174
TATACTG79000.016.2885255
GTACGAC23800.016.0201553
GTATTAT84050.015.5946051
GTATTAG50750.015.378881
GTATAAG49800.015.3133451
GTATAGG38850.014.7220951
CTAGACT55250.014.6647114
GTGTACG28850.014.6622951
TATACAG77500.014.5283155
ATAATAC80600.014.4132943
TAATACT92400.014.2490914
GTATAGA56550.014.222981
GTATATA94200.013.78781
CCTATAC39950.013.5702633
CCTAGAC60200.013.55775453
CTTACAC78150.013.4929823
GTATATG71500.013.4155741
GTATAAA111550.013.2990061
TAGACAG94900.012.9317155
Produced by FastQC (version 0.11.5)