FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20354698
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT50450.017.2369484
TATACTG77450.015.9183235
GTATATA94700.014.96972751
GTATTAG52150.014.6198381
TATACAG92350.014.1239325
CTAGACT57200.014.0574474
CTAGGAC67550.013.9315823
GTATTAC51400.013.7902061
CCTAGAC60800.013.7149163
CTACACT67850.013.6944154
TAATACT93650.013.610524
GTATAGG37350.013.55549051
GTATAAG54450.013.4553231
TAGACAG91450.013.4162745
TCTATAC52400.013.41283
ATAATAC79950.013.4098143
GTACTAG29450.013.3489031
TACACTG97300.013.2829735
GTATTAT83000.013.2046411
TAGACTG77600.013.04774955
Produced by FastQC (version 0.11.5)