FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15328600
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG56250.017.9819775
GTATTAG37500.017.2974221
CTAGACT44500.016.4459514
GTGTATA49250.014.7414341
TATACCG6609.458745E-1114.42404655
TTATACT76250.014.3579324
GTATAGA39500.014.1617511
GTATATA69400.014.1485061
GTGTACG22300.014.1435091
CTAGGAC51650.014.05411153
GGACCGT25500.013.9998096
TATACAG57700.013.8178385
GTGTAGA56850.013.8175051
CCTAGAC45650.013.8158933
CCTATAC28850.013.4054523
TAGACAG71950.013.3966225
TACACTG73100.013.26726155
GTATAAA84800.013.2633171
TAATACT67750.013.1733394
GTATTAT62050.013.0431741
Produced by FastQC (version 0.11.5)