FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15328600
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN180890.11800816773873675No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG35950.018.0648671
TATACCG7400.016.101585
GTACTAG23200.015.1520421
CTATACT39550.015.0638194
TATACTG56250.015.039595
TACACTG71150.014.8206955
GTATTAT64750.014.6306791
GTATATA72100.014.54402351
CTATACG7401.8189894E-1214.4918014
GTATAAG39450.014.3477481
GCTATAC25800.014.0860393
GTCTAAT38850.013.955891
TATACTA39750.013.7886235
GTATTAA65000.013.7494411
GTATAGG26050.013.723051
GTGTACG21500.013.5789061
CTAGACT44850.013.54937654
TATACAG60700.013.5444265
GTATTAC39200.013.5272991
TCTAGAC46050.013.4549613
Produced by FastQC (version 0.11.5)