FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15294025
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT38700.017.5273134
TACACCG14700.017.0001775
TACACTG70100.015.7027315
TCTATAC37950.015.5219013
CTAGGAC48200.015.3072143
TATACAG59000.015.2281255
TTATACT72700.014.65008454
GTATATA66200.014.5610991
GTATAAG37000.014.3128261
TAGACAG74500.014.2961235
TATACCG7503.6379788E-1214.28014855
GTATTAG37800.014.1673231
TATACTA40900.014.1113945
TATACTG57350.014.115
GTATATG49400.014.092761
CCTATAC30950.014.0340723
CTAGACT44050.013.91274454
TAATACT68900.013.9036284
TATAGTG38200.013.7069495
GCTATAC27000.013.6631053
Produced by FastQC (version 0.11.5)