FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15351212
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT37850.017.9209444
TATACTG56250.016.6073045
GTATTAG39200.016.3937421
TACACTG72700.016.2869825
TAATACG9300.015.3550164
TCTATAC42250.015.3504723
CTACACT52200.015.046154
TATACAG63150.014.981175
GTATTAT62000.014.7798281
ATAATAC61650.014.7665923
GTATAAA89150.014.4169281
GTATATA71350.014.3441511
CTAGACT45500.014.12323954
GTCTAGG42500.014.0007541
CCTAGAC44350.013.952813
TACACCG14300.013.7309275
GTATAGG29450.013.5372311
CTAGACG10600.013.4718534
CTAGGAC50850.013.2223753
GTATAGA41050.013.19072151
Produced by FastQC (version 0.11.5)