FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9381074
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG24250.018.893151
TATACTG35950.017.8746975
TACACCG12850.015.7430775
CTACACT29950.015.6943774
GTATAGT20000.015.4703011
TAGACAG39750.015.4174985
GTATATA41800.015.3735041
TACGACA16050.015.1992664
CCTAGAC25700.015.048613
TTATACT46150.014.69761854
GTATTAG25400.014.5239031
CCTACAC34300.014.2244633
TATACAG32400.014.1403295
CTATACT24900.014.0982924
CTAGACA32150.014.0652174
GTATTAA42200.013.9588011
TTAGACT30150.013.8141624
TCTATAC25150.013.7216443
TAATACT43100.013.6669544
CTAATAC28650.013.4991013
Produced by FastQC (version 0.11.5)