FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13884722
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG52250.017.6506375
TATACAG52150.016.5435475
CTATACT33550.016.315934
TACACCG16900.015.8431225
TACACTG65250.015.6842185
CCTAGAC39800.015.2487283
GTATATA65050.015.1859311
TAGACAG61650.015.0558925
GTATAGA35200.015.046241
GTATTAG33950.014.7238081
GTATAAG34100.014.48452951
CTAATAC36750.014.2476043
TAAGACG13950.014.0752554
TCTATAC35000.013.5999863
CTACACT43550.013.5258194
GTATAGG27300.013.5148021
CTAGACT37600.013.4507854
TAATACT61600.013.4261234
GTATAAT48500.013.37412551
CTAGACA51700.013.2349974
Produced by FastQC (version 0.11.5)