FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13884722
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN165440.11915254767074197No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG32950.017.7204721
CTATACT35250.017.069774
GTATATA64800.016.917941
GTATAGG22900.016.9115161
TATACAG50450.015.2328055
TATACTG51000.014.9517195
GTATAGA38000.014.267981
GTGTTAG41450.013.9428611
CTAGACT37650.013.9246144
TCTATAC34350.013.8748133
TACACTG64650.013.8221035
GTCCTAG42350.013.7872411
ACTATAC28200.013.7318213
CCTAGAC36550.013.691653
TATACCG7907.2759576E-1213.5736535
GTCTAGG38700.013.5480381
GTATAAG33200.013.4594681
TAGACAG60850.013.4125485
CTACACT43750.013.3448444
TCTAGAC41800.013.254723
Produced by FastQC (version 0.11.5)