FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13857515
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT34700.018.8622064
TATACTG54150.017.5812915
TATACAG52950.017.1931215
CCTATAC28550.015.6309143
GTATATA66050.015.5855781
GTATAAG35150.015.2358541
GTATAGA35100.015.0880281
TCTATAC36100.014.999043
CTAGACT38900.014.6842194
CTTACAC51800.014.4733833
GTGTACG20400.014.2927411
TACACAG82500.014.2082815
GTACTAG20700.014.08561
TAGACCG9350.014.0003965
CTAGGAC41800.013.9501553
TCTAGAC41050.013.9151313
GTATTAA56100.013.6828461
TAGGACC41600.013.4450924
CTACACT44600.013.3411854
TTATACT68350.013.3193244
Produced by FastQC (version 0.11.5)