FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13916369
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG6500.019.2458295
GTATAGG24400.019.0444451
GTATATA66100.017.4849261
GTATTAG35800.016.1418111
TACACTG67400.015.2903315
CTATACT33250.015.2291884
GTATAAG36650.014.7921371
GTCTTAA55750.014.6399381
CTAGACT38500.014.54509454
TCTATAC37750.014.5184523
TATACTG53350.014.5157535
CTACACT49800.014.4745644
GTCCTAC37050.014.1500511
GTCTTAG39000.013.9008171
GTATTAA60200.013.8546351
GTGCTAG39750.013.7884111
GTATAAC27050.013.65488151
TAGGGTG38500.013.6160965
CTAATAC40400.013.418673
CCTATAC26650.013.4123083
Produced by FastQC (version 0.11.5)