FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19480288
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT49050.017.602214
TATACTG77400.016.5398375
GTATAAG49750.016.0374931
GTGTACG31000.015.7498681
GTATAGG37800.015.594381
GTCTAGG58350.015.3064761
GTATATA90600.015.2470051
GTCCTAG61450.015.1156721
TATACAG76700.014.9052625
CTAGGAC70500.014.7803943
CCTAGAC65350.014.3051083
GTACGAC21800.013.9299883
TACACTG98500.013.9035375
GTACTAG31100.013.7846861
CTAGACT60250.013.6382994
TAATACT89250.013.6100644
GTATTAG48200.013.5884651
CCTACAC83350.013.5732893
CTACACT71600.013.3890914
TATACCG9400.013.3022515
Produced by FastQC (version 0.11.5)