FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19317215
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG9750.017.6971325
GTATTAG49200.016.6950451
GTATAGG39450.016.294851
GTACGAC20500.016.2534223
GTATATA94200.016.0493131
GTATAGA55600.015.7367911
TACACTG98750.015.6655525
CTACACT69600.015.6441684
GTATAGC36900.015.485261
GTACTAG33600.015.4118091
GTCTAGG62100.015.1439491
GTCCTAG63000.015.1165631
GTGTAGA89350.014.9886181
GTATTAT79300.014.9366621
GTATAAG52900.014.6272041
GTGTACG30150.014.6089181
GTATTAA84750.014.6082181
GTACTAA44650.014.53043651
GTCTAGA62150.014.461371
GTGCTAG66250.014.3749961
Produced by FastQC (version 0.11.5)