FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8709274
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG27700.017.8419725
TAGTACG3901.1651355E-716.7951034
GTATATA28300.016.2011681
CTACACT30950.015.9687894
TACACCG9350.015.9210965
TATACCG5109.669748E-915.1781115
CTATACT18450.015.1689694
GTATTAC18200.015.0497231
CTAGACT22000.014.8865684
TTATACT32250.014.5865294
GTATTAA30950.014.4292131
GTATTAG18300.014.3167231
GTATAGG15950.014.1861631
TCTATAC19500.014.0468123
GTACTAG10350.013.8074331
GTCTTAC26750.013.8009811
TAGACCG5653.7225618E-813.7005965
GTACTAT16550.013.6718621
TATACGT4801.2253968E-613.6457055
CCTAGAC23300.013.544863
Produced by FastQC (version 0.11.5)