FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8640186
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG5555.456968E-1217.1711484
GTATTAG19150.016.1731381
GTCCTAG25450.015.9140551
CTAGTAC11600.015.4039853
TAGACCG6605.456968E-1215.3417465
GTATTAT28500.015.0469111
GTATAGA20600.015.0347391
CTATACT17850.014.6820544
TAATACT31050.014.5788864
GTATATA27050.014.0919931
GTGCTAG25200.013.9447651
GTCTATA16850.013.7855621
GTACTAG10000.013.6989591
ATAATAC28700.013.6972023
TAGACAG34400.013.67855
GTCCTAA20050.013.6647971
GTATTAA27150.013.6013371
GTCTAGG24150.013.5645481
GTGTACG13200.013.5365211
CTACACT30300.013.3671454
Produced by FastQC (version 0.11.5)