FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8642029
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG19750.018.081771
GTCTAGG27550.017.283241
GTATATA29400.015.7907961
GTATTAA28150.015.4348091
GTATTAC19000.015.3496771
GTGTAGG35750.015.3168171
GTCCTAT19850.015.2920761
CTACACT27300.015.2562014
GTGTACG13050.015.0508211
CTATACT18200.015.0382564
GTCTTAG23750.014.7858111
GTATTAT29000.014.777171
GTGTAGA35250.014.6898351
GTCTAGA22800.014.618741
GTATTAG17250.014.4916211
GTATAGC14850.014.4288861
GTATAGA20700.014.3766091
TACACTG39350.014.3644795
CTAATAC20750.014.33715253
GTATAGG15650.014.0716221
Produced by FastQC (version 0.11.5)