FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26820739
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG12650.017.8910685
CTATACT80900.017.080054
TATACTG120050.016.3717445
GTATACG15700.016.3133981
GTATAGG57800.015.5605421
GTATATA155900.015.2059151
TACACTG149950.014.77541355
GTATAAG77600.014.7372051
CTACACT109400.014.5903994
GTATAGA88700.014.3702981
CTAGGAC98400.014.2845753
TCTATAC83750.013.8675673
GTATTAT129850.013.8528981
TATACAG126450.013.8475085
GTCTAGG93500.013.7599791
GTATTAG76950.013.7006221
TAATACT136150.013.6922994
CCTAGAC83750.013.654223
TAGACTG111150.013.5031235
GTATTAA127100.013.3559551
Produced by FastQC (version 0.11.5)