FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26907491
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG118350.016.6915575
GTATAGA82650.016.5595441
CTATACT78250.016.5007024
GTATAAG78000.016.0972331
TATACAG126450.015.7164585
TACACTG146350.015.6529085
GTATTAG78000.015.4106211
GTATATA162500.015.27024651
CTACACT104850.014.9817494
GTATAGG58100.014.9534031
GTCCTAG94200.014.90817451
TCTATAC83150.014.8127863
GTATTAT129550.014.0555341
GTGTAGG116300.013.86605551
GTGTATA114250.013.8544351
CCTAGAC87800.013.7572043
TAGACTG110950.013.6753975
CTAGGAC102900.013.6466263
CCTATAC57900.013.5651313
TACACAG197950.013.5565165
Produced by FastQC (version 0.11.5)