FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27026029
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG56650.017.6461871
TATACTG122050.017.2096485
GTATTAG79900.016.905211
TATACAG116200.016.8982945
TACACTG148750.016.6806965
GTATAAG78300.016.3387261
GTATAGA87750.016.1388031
GTGTACG42700.015.8861441
CTATACT80050.015.6096744
GTATATA159400.015.6037671
CTACACT98900.015.4020874
GTATTAA132550.015.352811
GTATTAT132950.015.2171071
GTCCTAG94800.014.436471
GTATACG14600.014.2645041
GTATAAT123700.014.1903721
CCTATAC59750.014.141283
GTCTTAG86950.014.028971
TCTAGAC89100.013.6235653
TAATACT142550.013.5660074
Produced by FastQC (version 0.11.5)