FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27026029
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG53350.016.3000371
TATACAG119800.016.1082635
TATACTG114350.015.7821795
CTATACT80250.015.3637734
GTATTAG81600.015.3284871
TATACCG14750.014.9406755
GTATATA160900.014.9182951
GTATAGA84550.014.7936681
TACACTG147000.014.7483875
TCTATAC84900.014.3118273
TAGACAG142450.014.2996085
CTACACT105900.014.285994
GTATAAG80550.014.1233591
GTCCTAG94900.013.7451061
GTCTAGG89950.013.7069031
TAATACT136350.013.5855694
CCTACAC113250.013.3588213
GTGTAGG126250.013.3513521
GTGTTAG99700.013.322321
CCTATAC59550.013.3027823
Produced by FastQC (version 0.11.5)