FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26541626
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG125100.017.0907755
CTATACT80050.015.6237414
GTATATA163550.015.2954081
GTATAGA90300.015.23654651
GTATAGG59100.015.2177961
TAGACAG142500.014.6277595
TACACTG145800.014.6234595
GTATTAG79600.014.5161341
GTATAAG76450.014.3351631
CTACACT104700.014.1069154
GTATTAT133200.014.08538151
TATACCG14050.013.9882115
TATACAG126300.013.9105175
TCTATAC89050.013.8441123
CCTACAC117400.013.6969663
CCTAGAC89100.013.6358173
GTATACG15650.013.3203611
TTATACT156350.013.2939674
TAATACT144200.013.1337554
GTCCTAG94300.013.0112141
Produced by FastQC (version 0.11.5)