FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26845635
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG117900.017.7647785
CTATACT87550.017.05884
GTATAGA88700.015.9658911
GTACTAG45900.015.9452831
GTATTAG78100.015.7709961
CTACACT109350.015.6168724
GTGTACG45200.015.5340031
GTATATA170750.015.4377091
GTATAGG58650.015.3196481
TCTATAC84650.014.9018653
GTATAAG78000.014.8757831
TATACAG125100.014.5544245
CTAGACT90150.014.520714
GTACGAC30100.014.4307233
TAGACTG115200.014.4106195
TATACGG15750.014.3560165
TAGACAG146750.014.272355
TACACTG148450.014.2291545
GTATTAT133700.014.1525771
TAATACT147200.013.8244934
Produced by FastQC (version 0.11.5)