FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16838087
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG65850.017.0780335
GTATAGG31600.016.947741
GTATTAG42650.016.6029131
GTATAAG43250.016.3725831
CTATACT43600.015.9674334
CTAGACT46650.015.8163324
GTGTTAG54300.015.6708521
GTATATA83650.015.578821
TACACTG78750.015.4894215
CTAGTAC27300.015.4750753
GTATAGA47550.015.3925631
TACACCG14950.015.1242655
GTGTACG21400.014.7373181
GTATTAA72550.014.6815531
GTATAAA102400.014.5857951
GTATTAT69100.014.5534191
GTCTAGG49150.014.5282771
GTGTATA61450.014.525321
CCTAGAC49300.014.4834273
CTACACT55650.014.4345864
Produced by FastQC (version 0.11.5)