FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16929822
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA48350.017.118311
CTATACT42350.016.7316474
GTATTAG42150.016.5284021
CCTATAC34500.015.5334743
TATACTG65400.015.3867775
TAGACAG77500.014.9820645
CTAGACA64650.014.552374
TATACAG65600.014.522955
GTATATA84150.014.5058211
GTATATG60750.014.408331
GTCTAGG48100.014.3600361
CTAGACT46400.014.2445974
GTCTTAC52900.014.0700921
GTATAAA96450.013.9523951
TACACCG15600.013.7409465
CTATAGT40750.013.735534
TATACCG8251.8189894E-1213.7131865
GTGCTAG52200.013.688421
TCTATAC42950.013.5865143
CTAGGAC54050.013.5504773
Produced by FastQC (version 0.11.5)