FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14516130
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGG52600.017.8740791
GTATAGG24150.017.4941771
GTATAAG34200.016.007151
TATACAG56400.015.719715
TACACCG13750.015.5789145
TACACTG63050.015.4773335
TAGACAG64450.015.2334555
CCTAGAC39700.015.1383563
GTATTAG33750.014.9864061
CTATACT31300.014.8282764
GTACTAG19500.014.64734651
GTCTAGA40300.014.47016051
GTATAGA37450.014.300231
GTATTAA55500.014.2597211
TATACTG54050.014.0913195
CTAGACA53350.014.053244
GTCCTAG42150.013.9762281
TAGACTG57700.013.9217985
CGTACAC17550.013.9012733
CTAGGAC47200.013.7414443
Produced by FastQC (version 0.11.5)