FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14532169
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC38000.016.611863
GTATATA65850.016.4591961
CTATACG6251.8189894E-1216.1981334
GTATTAG34750.015.7661651
GTGTACG20650.015.5728011
TATACCG5751.8007995E-1015.5352395
TATACTG54050.015.425065
GTATAAG36700.015.4152491
CTATACT32850.015.4091834
CTAGACT37250.015.3476194
CTACACT49300.015.2201764
TACACTG67700.015.1298385
TATACAG52650.014.7041365
GTCTTAG41900.014.6391631
GTACTAA29300.014.4305781
GTATTAA55550.014.365271
CTTACAC53700.014.3057783
GTATTAC36300.014.2726851
GTCTAGG39100.014.0121291
GTATAGA36550.014.0121281
Produced by FastQC (version 0.11.5)