FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10520963
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG15050.016.6057361
TATACCG5551.07320375E-1016.0814425
CTAGACT27350.015.6639724
GTATTAA33300.015.0099891
TAATACT40850.015.0027864
CTATACT22100.014.8080254
TATACTG33850.014.5896945
TACACTG42250.014.5056525
GTGTAGA34550.014.4669361
TATACAG32600.014.4190375
GTATTAT35250.014.1796481
CTAGGAC31950.014.1536843
GTATAAG22000.014.0645551
TAGGCCG10650.013.967455
GTCTAAG21000.013.884241
GTATATA40300.013.8793191
TAGACTG37400.013.6821275
TAGACAG39150.013.6784695
GACTGTG70600.013.6525697
GTACGAC10050.013.6172223
Produced by FastQC (version 0.11.5)