FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10520963
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN115280.10957171886261743No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG22800.017.5023141
GTACGAC10600.017.4180263
GTCCTAG28000.015.7408871
GGACCGT16100.015.5303166
TAGACAG40100.015.446575
CCTAGAC25450.015.4454033
CTATACT19850.015.3021614
TAGGACA31600.015.2665544
CTAGGAC29700.015.2405373
CTAGACT24600.015.0106574
GTACTAG13350.014.7227251
TAGGACG6901.2732926E-1114.6738124
CTTACAC33700.014.6686893
GTATAGA22400.014.6241361
TACACTG41650.014.5857335
GTATTAC19950.014.3302531
CTAGACA34150.014.3009954
GTCTACG11250.014.2944281
ATAGGAC24600.014.2843353
GTATTAA35550.014.2408151
Produced by FastQC (version 0.11.5)