FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10563241
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG4351.1841621E-917.7818285
GTATAGA22450.017.4928341
TAGACAG43450.017.3914685
GTGTACG13300.017.000611
CTATACT20350.016.081284
CCTAGAC26250.015.866863
TACACTG45800.015.3298835
GTATATA42050.015.2823571
TATACTG34200.015.1361515
TACGACA12600.015.1112984
CTAGACA33450.014.5861274
TTATACT43300.014.5659984
CTAGACT29400.014.3692244
TCTATAC24850.014.3663743
TTACGGT8350.014.2516734
GTATAGG14300.014.1473631
TAGACTG39100.014.0001735
TACGCAG11150.013.874615
TATACTA21900.013.8563365
TATAGTG21500.013.8373795
Produced by FastQC (version 0.11.5)